- Three hidden levels, reached through rare entrances: Level 37 "The Poolrooms", Level Fun =) and Level ! "Run For Your Life"
- New entities: Smilers, Skin-Stealers (they wear a teammate's name in co-op), Partygoers, the Thing on Level 7 and Mimicry
- Credits tab crediting every Backrooms Wiki author whose work is adapted
- Almond Water (Level 4): restores thirst and sanity
- Content relating to the Backrooms is licensed under Creative Commons Attribution-ShareAlike 3.0 and all concepts originate from backrooms-wiki.wikidot.com and its authors. Pixel Backrooms, being derived from this content, is also released under CC BY-SA 3.0.
- Level 3: iron bars, scalding sludge and breakers that black out the lights
- Level 4: windows that aren't blacked out are traps, don't look outside
- Level 6: light doesn't work at all, follow the sound of waves to the stairs
- Level 7: a dark, freezing sea with narrow walkways
- Level 9: suburb streets at night, fog rolls in and the hunters get bolder
- Levels 10 and 11 renamed to match the wiki: "Bumper Crop" and "The City That Never Sleeps"
- Hounds hesitate while you hold their gaze
- Louder footsteps
- You could see through walls when standing right against them
- Hiding under a desk put your view on top of the desk
- Lockers now have slats you can look out through
- You can climb out of deep water (face the edge and press Space)
- Co-op has only been tested on one PC so far